Measurement

Part:BBa_K549027:Design

Designed by: Franziska Haefele   Group: iGEM11_LMU-Munich   (2011-09-16)


10x His-tag fused to petE2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal PstI site found at 236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 236
    Illegal NotI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 434
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal PstI site found at 236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal PstI site found at 236
    Illegal NgoMIV site found at 23
    Illegal AgeI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The 10X His-tag originates from BBa_K549019 and petE2 from BBa_K549024.

References